NFIB Kit ELISA

N° du produit ABIN5657442

Détail du produit NFIB Kit ELISA

Antigène: NFIB (Nuclear Factor I/B (NFIB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Nuclear Factor I/B (NFIB). No significant cross-reactivity or interference between Nuclear Factor I/B (NFIB) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor I/B (NFIB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor I/B (NFIB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor I/B (NFIB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Factor I/B (NFIB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor I/B (NFIB) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor I/B (NFIB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NFIB (Nuclear Factor I/B (NFIB))

Autre désignation: Nuclear Factor I/B (NFIB Produits)

Synonymes: NFIB Kit ELISA, nf1-b1 Kit ELISA, CTF Kit ELISA, HMGIC/NFIB Kit ELISA, NF-I/B Kit ELISA, NF1-B Kit ELISA, NFI-B Kit ELISA, NFI-RED Kit ELISA, NFIB2 Kit ELISA, NFIB3 Kit ELISA, 6720429L07Rik Kit ELISA, E030026I10Rik Kit ELISA, nuclear factor I B Kit ELISA, nuclear factor 1 B-type Kit ELISA, nuclear factor I B L homeolog Kit ELISA, nuclear factor I/B Kit ELISA, NFIB Kit ELISA, LOC100221557 Kit ELISA, nfib.L Kit ELISA, Nfib Kit ELISA

Sujet:

Gene Name: Nuclear Factor I/B

Gene Aliases: NFI-RED, NFIB2, NFIB3, CCAAT-box-binding transcription factor, TGGCA-binding protein

ID gène: 4781

UniProt: O00712