NCOA3 Kit ELISA

N° du produit ABIN5657459

Détail du produit NCOA3 Kit ELISA

Antigène: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Nuclear Receptor Coactivator 3 (NCOA3). No significant cross-reactivity or interference between Nuclear Receptor Coactivator 3 (NCOA3) and analogues was observed.

Sensibilité: 0.124 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Coactivator 3 (NCOA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Receptor Coactivator 3 (NCOA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Receptor Coactivator 3 (NCOA3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Receptor Coactivator 3 (NCOA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Autre désignation: Nuclear Receptor Coactivator 3 (NCOA3 Produits)

Synonymes: NCOA3 Kit ELISA, ACTR Kit ELISA, AIB-1 Kit ELISA, AIB1 Kit ELISA, CAGH16 Kit ELISA, CTG26 Kit ELISA, KAT13B Kit ELISA, RAC3 Kit ELISA, SRC-3 Kit ELISA, SRC3 Kit ELISA, TNRC14 Kit ELISA, TNRC16 Kit ELISA, TRAM-1 Kit ELISA, bHLHe42 Kit ELISA, pCIP Kit ELISA, 2010305B15Rik Kit ELISA, AW321064 Kit ELISA, Actr Kit ELISA, Aib1 Kit ELISA, Rac3 Kit ELISA, Src3 Kit ELISA, Tram-1 Kit ELISA, Tram1 Kit ELISA, p/Cip Kit ELISA, pCip Kit ELISA, actr Kit ELISA, aib-1 Kit ELISA, aib1 Kit ELISA, cagh16 Kit ELISA, ctg26 Kit ELISA, kat13b Kit ELISA, pcip Kit ELISA, rac3 Kit ELISA, src-3 Kit ELISA, src3 Kit ELISA, tnrc14 Kit ELISA, tnrc16 Kit ELISA, tram-1 Kit ELISA, xSRC-3 Kit ELISA, nuclear receptor coactivator 3 Kit ELISA, nuclear receptor coactivator 3 S homeolog Kit ELISA, NCOA3 Kit ELISA, Ncoa3 Kit ELISA, ncoa3.S Kit ELISA

Sujet:

Gene Name: Nuclear Receptor Coactivator 3

Gene Aliases: RAC3, ACTR, AIB1, CAGH16, CTG26, SRC3, TNRC14, TNRC16, TRAM1, pCIP, Amplified In Breast 1, Steroid Receptor Coactivator-3 , Thyroid Hormone Receptor Activator Molecule 1

ID gène: 8202

UniProt: Q9Y6Q9

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha