NAP1L1 Kit ELISA

N° du produit ABIN5657477

Détail du produit NAP1L1 Kit ELISA

Antigène: NAP1L1 (Nucleosome Assembly Protein 1-Like 1 (NAP1L1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1). No significant cross-reactivity or interference between Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1) and analogues was observed.

Sensibilité: 0.119 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NAP1L1 (Nucleosome Assembly Protein 1-Like 1 (NAP1L1))

Autre désignation: Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1 Produits)

Synonymes: NAP1 Kit ELISA, NAP1L Kit ELISA, NRP Kit ELISA, cb716 Kit ELISA, sb:cb13 Kit ELISA, wu:fb83g07 Kit ELISA, nap1-B Kit ELISA, nap1l1 Kit ELISA, xNAP-1 Kit ELISA, xNAP1L-B Kit ELISA, xnap1l Kit ELISA, xnap1l1 Kit ELISA, nap1 Kit ELISA, nap1l Kit ELISA, nrp Kit ELISA, AA407126 Kit ELISA, AI256722 Kit ELISA, D10Ertd68e Kit ELISA, NAP-1 Kit ELISA, ARABIDOPSIS THALIANA NUCLEOSOME ASSEMLY PROTEIN 1;1 Kit ELISA, ATNAP1;1 Kit ELISA, F20B18.220 Kit ELISA, F20B18_220 Kit ELISA, nucleosome assembly protein1;1 Kit ELISA, nap1-A Kit ELISA, xNAP1 Kit ELISA, xNAP1L-A Kit ELISA, nucleosome assembly protein 1 like 1 Kit ELISA, nucleosome assembly protein 1-like 1 Kit ELISA, nucleosome assembly protein 1 like 1 L homeolog Kit ELISA, nucleosome assembly protein1;1 Kit ELISA, nucleosome assembly protein 1 like 1 S homeolog Kit ELISA, NAP1L1 Kit ELISA, nap1l1 Kit ELISA, nap1l1.L Kit ELISA, Nap1l1 Kit ELISA, NAP1;1 Kit ELISA, nap1l1.S Kit ELISA

Sujet:

Gene Name: Nucleosome Assembly Protein 1 Like Protein 1

Gene Aliases: NAP1, NAP1L, NRP, NAP-1-related protein

ID gène: 4673

UniProt: P55209