OAT Kit ELISA

N° du produit ABIN5657518

Détail du produit OAT Kit ELISA

Antigène: OAT (Ornithine Aminotransferase (OAT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.625 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.625 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ornithine Aminotransferase (OAT). No significant cross-reactivity or interference between Ornithine Aminotransferase (OAT) and analogues was observed.

Sensibilité: 0.253 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Aminotransferase (OAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Aminotransferase (OAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Aminotransferase (OAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ornithine Aminotransferase (OAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ornithine Aminotransferase (OAT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ornithine Aminotransferase (OAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: OAT (Ornithine Aminotransferase (OAT))

Autre désignation: Ornithine Aminotransferase (OAT Produits)

Synonymes: 151832_at Kit ELISA, CG8782 Kit ELISA, Dmel\\CG8782 Kit ELISA, hoga Kit ELISA, AI194874 Kit ELISA, rOAT Kit ELISA, GACR Kit ELISA, HOGA Kit ELISA, OATASE Kit ELISA, OKT Kit ELISA, Ornithine aminotransferase precursor Kit ELISA, ornithine aminotransferase, gene 1 Kit ELISA, ornithine aminotransferase Kit ELISA, Oat Kit ELISA, oat.1 Kit ELISA, OAT Kit ELISA, Lbys_2951 Kit ELISA, Riean_0998 Kit ELISA, Ftrac_2019 Kit ELISA, Celal_2605 Kit ELISA, Deipr_1149 Kit ELISA, Celly_0230 Kit ELISA, Weevi_2065 Kit ELISA, Fluta_4071 Kit ELISA, Halhy_2848 Kit ELISA, Theth_0565 Kit ELISA, oat Kit ELISA

Sujet:

Gene Name: Ornithine Aminotransferase

Gene Aliases: HOGA, Grate Arophy, Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase

ID gène: 4942

UniProt: P04181