Ovalbumin Kit ELISA

N° du produit ABIN5657567

Détail du produit Ovalbumin Kit ELISA

Antigène: Ovalbumin (OVA)

Reactivité: Différentes espèces

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 123.5 pg/mL - 10000 pg/mL

Seuil minimal de détection: 123.5 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ovalbumin (OVA). No significant cross-reactivity or interference between Ovalbumin (OVA) and analogues was observed.

Sensibilité: 52.4 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 h

Plaque: Pre-coated

Protocole: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ovalbumin (OVA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ovalbumin (OVA) and unlabeled Ovalbumin (OVA) (Standards or samples) with the pre-coated antibody specific to Ovalbumin (OVA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ovalbumin (OVA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ovalbumin (OVA) in the sample.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ovalbumin (OVA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ovalbumin (OVA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Ovalbumin (OVA)

Autre désignation: Ovalbumin (OVA Produits)

Synonymes: 1600019A21Rik Kit ELISA, R86 Kit ELISA, SPI-CI Kit ELISA, Spi10 Kit ELISA, ovalbumin Kit ELISA, NK13 Kit ELISA, Spi12 Kit ELISA, BC052216 Kit ELISA, NK21 Kit ELISA, NK21L1 Kit ELISA, Spi13 Kit ELISA, NK26 Kit ELISA, Spi14 Kit ELISA, AW540195 Kit ELISA, AT2 Kit ELISA, Spi9 Kit ELISA, EID Kit ELISA, SPI3B Kit ELISA, Gm11396 Kit ELISA, OTTMUSG00000000720 Kit ELISA, SERPINB14 Kit ELISA, C76171 Kit ELISA, C76174 Kit ELISA, SPI3C Kit ELISA, SPIC Kit ELISA, serine (or cysteine) peptidase inhibitor, clade B, member 9b Kit ELISA, serine (or cysteine) peptidase inhibitor, clade B, member 6b Kit ELISA, serine (or cysteine) peptidase inhibitor, clade B, member 9f Kit ELISA, serine (or cysteine) peptidase inhibitor, clade B, member 9e Kit ELISA, serine (or cysteine) peptidase inhibitor, clade B, member 9d Kit ELISA, serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene Kit ELISA, serine (or cysteine) peptidase inhibitor, clade B, member 6e Kit ELISA, ovalbumin (SERPINB14) Kit ELISA, serine (or cysteine) peptidase inhibitor, clade B, member 6c Kit ELISA, Serpinb9b Kit ELISA, Serpinb6b Kit ELISA, Serpinb9f Kit ELISA, Serpinb9e Kit ELISA, Serpinb9d Kit ELISA, Serpinb1-ps1 Kit ELISA, Serpinb6e Kit ELISA, OVAL Kit ELISA, Serpinb6c Kit ELISA

Classe de substances: Virus

Sujet:

Gene Name: Ovalbumin