OGG1 Kit ELISA

N° du produit ABIN5657583

Détail du produit OGG1 Kit ELISA

Antigène: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Oxoguanine Glycosylase 1 (OGG1). No significant cross-reactivity or interference between Oxoguanine Glycosylase 1 (OGG1) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxoguanine Glycosylase 1 (OGG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oxoguanine Glycosylase 1 (OGG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxoguanine Glycosylase 1 (OGG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Oxoguanine Glycosylase 1 (OGG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxoguanine Glycosylase 1 (OGG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxoguanine Glycosylase 1 (OGG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Autre désignation: Oxoguanine Glycosylase 1 (OGG1 Produits)

Synonymes: OGG1 Kit ELISA, zgc:158858 Kit ELISA, 8-oxoguanine-DNA glycosylase 1 Kit ELISA, ARABIDOPSIS 8-OXOGUANINE-DNA GLYCOSYLASE 1 Kit ELISA, ATOGG1 Kit ELISA, F8K7.14 Kit ELISA, F8K7_14 Kit ELISA, 19.m02329 Kit ELISA, DDBDRAFT_0184479 Kit ELISA, DDBDRAFT_0233024 Kit ELISA, DDB_0184479 Kit ELISA, DDB_0233024 Kit ELISA, ogg1 Kit ELISA, Tb04.1H19.930 Kit ELISA, HMMH Kit ELISA, HOGG1 Kit ELISA, MUTM Kit ELISA, OGH1 Kit ELISA, Mmh Kit ELISA, 8-oxoguanine DNA glycosylase Kit ELISA, 8-oxoguanine DNA glycosylase L homeolog Kit ELISA, 8-oxoguanine-DNA glycosylase 1 Kit ELISA, 8-OXOGUANINE DNA GLYCOSYLASE Kit ELISA, putative 8-oxoguanine DNA glycosylase Kit ELISA, 8-oxoguanine DNA-glycosylase Kit ELISA, N-glycosylase/DNA lyase OGG1 Kit ELISA, 8-oxoguanine DNA-glycosylase 1 Kit ELISA, OGG1 Kit ELISA, ogg1.L Kit ELISA, ogg1 Kit ELISA, ECU08_0770 Kit ELISA, CA_C2707 Kit ELISA, BBOV_I004290 Kit ELISA, Smp_074010.1 Kit ELISA, LOC9329243 Kit ELISA, Tb927.4.2480 Kit ELISA, Ogg1 Kit ELISA

Sujet:

Gene Name: Oxoguanine Glycosylase 1

Gene Aliases: HMMH, HOGG1, MUTM, OGH1, 8-Oxoguanine DNA Glycosylase, DNA-(apurinic or apyrimidinic site) lyase, N-glycosylase/DNA lyase

ID gène: 4968

UniProt: O15527

Pathways: Réparation de l'ADN