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OGG1 Kit ELISA

OGG1 Reactivité: Souris Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5657584
  • Antigène Voir toutes OGG1 Kits ELISA
    OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))
    Reactivité
    • 3
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Oxoguanine Glycosylase 1 (OGG1). No significant cross-reactivity or interference between Oxoguanine Glycosylase 1 (OGG1) and analogues was observed.
    Sensibilité
    12.7 pg/mL
    Top Product
    Discover our top product OGG1 Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxoguanine Glycosylase 1 (OGG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oxoguanine Glycosylase 1 (OGG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxoguanine Glycosylase 1 (OGG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Oxoguanine Glycosylase 1 (OGG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxoguanine Glycosylase 1 (OGG1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxoguanine Glycosylase 1 (OGG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes OGG1 Kits ELISA
    OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))
    Autre désignation
    Oxoguanine Glycosylase 1 (OGG1 Produits)
    Synonymes
    OGG1 Kit ELISA, zgc:158858 Kit ELISA, 8-oxoguanine-DNA glycosylase 1 Kit ELISA, ARABIDOPSIS 8-OXOGUANINE-DNA GLYCOSYLASE 1 Kit ELISA, ATOGG1 Kit ELISA, F8K7.14 Kit ELISA, F8K7_14 Kit ELISA, 19.m02329 Kit ELISA, DDBDRAFT_0184479 Kit ELISA, DDBDRAFT_0233024 Kit ELISA, DDB_0184479 Kit ELISA, DDB_0233024 Kit ELISA, ogg1 Kit ELISA, Tb04.1H19.930 Kit ELISA, HMMH Kit ELISA, HOGG1 Kit ELISA, MUTM Kit ELISA, OGH1 Kit ELISA, Mmh Kit ELISA, 8-oxoguanine DNA glycosylase Kit ELISA, 8-oxoguanine DNA glycosylase L homeolog Kit ELISA, 8-oxoguanine-DNA glycosylase 1 Kit ELISA, 8-OXOGUANINE DNA GLYCOSYLASE Kit ELISA, putative 8-oxoguanine DNA glycosylase Kit ELISA, 8-oxoguanine DNA-glycosylase Kit ELISA, N-glycosylase/DNA lyase OGG1 Kit ELISA, 8-oxoguanine DNA-glycosylase 1 Kit ELISA, OGG1 Kit ELISA, ogg1.L Kit ELISA, ogg1 Kit ELISA, ECU08_0770 Kit ELISA, CA_C2707 Kit ELISA, BBOV_I004290 Kit ELISA, Smp_074010.1 Kit ELISA, LOC9329243 Kit ELISA, Tb927.4.2480 Kit ELISA, Ogg1 Kit ELISA
    Sujet

    Gene Name: Oxoguanine Glycosylase 1

    Gene Aliases: HMMH, HOGG1, MUTM, OGH1, 8-Oxoguanine DNA Glycosylase, DNA-(apurinic or apyrimidinic site) lyase, N-glycosylase/DNA lyase

    ID gène
    18294
    UniProt
    O08760
    Pathways
    Réparation de l'ADN
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