PAX6 Kit ELISA

N° du produit ABIN5657602

Détail du produit PAX6 Kit ELISA

Antigène: PAX6 (Paired Box 6 (PAX6))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Paired Box Gene 6 (PAX6). No significant cross-reactivity or interference between Paired Box Gene 6 (PAX6) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paired Box Gene 6 (PAX6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paired Box Gene 6 (PAX6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paired Box Gene 6 (PAX6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paired Box Gene 6 (PAX6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Paired Box Gene 6 (PAX6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Paired Box Gene 6 (PAX6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PAX6 (Paired Box 6 (PAX6))

Autre désignation: Paired Box Gene 6 (PAX6 Produits)

Synonymes: pax6 Kit ELISA, an2 Kit ELISA, mgda Kit ELISA, wagr Kit ELISA, pax-6 Kit ELISA, pax6a Kit ELISA, xpax6 Kit ELISA, XLPAX6 Kit ELISA, MGC52531 Kit ELISA, PAX-6 Kit ELISA, AN Kit ELISA, AN2 Kit ELISA, D11S812E Kit ELISA, MGDA Kit ELISA, WAGR Kit ELISA, Pax-6 Kit ELISA, pax6b Kit ELISA, 1500038E17Rik Kit ELISA, AEY11 Kit ELISA, Dey Kit ELISA, Gsfaey11 Kit ELISA, Sey Kit ELISA, PAXZF-A Kit ELISA, Pax6.1 Kit ELISA, cb280 Kit ELISA, fc20e07 Kit ELISA, pax-a Kit ELISA, pax[zf-a] Kit ELISA, paxzfa Kit ELISA, wu:fc20e07 Kit ELISA, zfpax-6a Kit ELISA, zfpax-6b Kit ELISA, paired box 6 Kit ELISA, paired box 6 L homeolog Kit ELISA, paired box 6 S homeolog Kit ELISA, paired box 6a Kit ELISA, PAX6 Kit ELISA, pax6 Kit ELISA, Pax6 Kit ELISA, pax6.L Kit ELISA, pax6.S Kit ELISA, pax6a Kit ELISA

Sujet:

Gene Name: Paired Box Gene 6

Gene Aliases: AN, AN2, MGDA, WAGR, Oculorhombin, Aniridia,Keratitis, Aniridia type II protein

ID gène: 25509

UniProt: P63016

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis