PDE4B Kit ELISA

N° du produit ABIN5657767

Détail du produit PDE4B Kit ELISA

Antigène: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 4B, cAMP Specific (PDE4B). No significant cross-reactivity or interference between Phosphodiesterase 4B, cAMP Specific (PDE4B) and analogues was observed.

Sensibilité: 0.065 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 4B, cAMP Specific (PDE4B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 4B, cAMP Specific (PDE4B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 4B, cAMP Specific (PDE4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 4B, cAMP Specific (PDE4B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphodiesterase 4B, cAMP Specific (PDE4B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphodiesterase 4B, cAMP Specific (PDE4B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Autre désignation: Phosphodiesterase 4B, cAMP Specific (PDE4B Produits)

Synonymes: dpde4 Kit ELISA, pde4b5 Kit ELISA, pdeivb Kit ELISA, Dpde4 Kit ELISA, R74983 Kit ELISA, dunce Kit ELISA, PDE4/IVb Kit ELISA, Pde4B1 Kit ELISA, Pde4B2 Kit ELISA, Pde4B3 Kit ELISA, Pde4B4 Kit ELISA, DPDE4 Kit ELISA, PDE4B5 Kit ELISA, PDEIVB Kit ELISA, phosphodiesterase 4B Kit ELISA, phosphodiesterase 4B, cAMP specific Kit ELISA, phosphodiesterase 4B S homeolog Kit ELISA, pde4b Kit ELISA, PDE4B Kit ELISA, Pde4b Kit ELISA, pde4b.S Kit ELISA

Sujet:

Gene Name: Phosphodiesterase 4B, cAMP Specific

Gene Aliases: DPDE4, PDE32, Dunce(Drosophila)Homolog Phosphodiesterase E4, cAMP-specific 3',5'-cyclic phosphodiesterase 4B

ID gène: 24626

UniProt: P14646

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction