PTTG1IP Kit ELISA

N° du produit ABIN5657835

Détail du produit PTTG1IP Kit ELISA

Antigène: PTTG1IP (Pituitary Tumor-Transforming 1 Interacting Protein (PTTG1IP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP). No significant cross-reactivity or interference between Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) and analogues was observed.

Sensibilité: 0.071 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PTTG1IP (Pituitary Tumor-Transforming 1 Interacting Protein (PTTG1IP))

Autre désignation: Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP Produits)

Synonymes: 1810010L20Rik Kit ELISA, AI314311 Kit ELISA, AU018448 Kit ELISA, C79540 Kit ELISA, C21orf1 Kit ELISA, C21orf3 Kit ELISA, PBF Kit ELISA, pttg1ip Kit ELISA, zgc:103708 Kit ELISA, pituitary tumor-transforming 1 interacting protein Kit ELISA, PTTG1 interacting protein Kit ELISA, PTTG1 interacting protein b Kit ELISA, Pttg1ip Kit ELISA, PTTG1IP Kit ELISA, pttg1ipb Kit ELISA

Sujet:

Gene Name: Pituitary Tumor Transforming 1 Interacting Protein

Gene Aliases: PBF, C21orf1, C21orf3, Pituitary tumor-transforming gene protein-binding factor

ID gène: 754

UniProt: P53801

Pathways: Protein targeting to Nucleus