PAFAH2 Kit ELISA

N° du produit ABIN5657884

Détail du produit PAFAH2 Kit ELISA

Antigène: PAFAH2 (Platelet-Activating Factor Acetylhydrolase 2, 40kDa (PAFAH2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Platelet Activating Factor Acetylhydrolase 2 (PAFAH2). No significant cross-reactivity or interference between Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) and analogues was observed.

Sensibilité: 0.105 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Activating Factor Acetylhydrolase 2 (PAFAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PAFAH2 (Platelet-Activating Factor Acetylhydrolase 2, 40kDa (PAFAH2))

Autre désignation: Platelet Activating Factor Acetylhydrolase 2 (PAFAH2 Produits)

Synonymes: HSD-PLA2 Kit ELISA, hsd-pla2 Kit ELISA, PAFAH2 Kit ELISA, 2310074E22Rik Kit ELISA, AI507170 Kit ELISA, platelet activating factor acetylhydrolase 2 Kit ELISA, platelet-activating factor acetylhydrolase 2 Kit ELISA, platelet-activating factor acetylhydrolase 2 L homeolog Kit ELISA, PAFAH2 Kit ELISA, Pafah2 Kit ELISA, pafah2.L Kit ELISA, pafah2 Kit ELISA

Sujet:

Gene Name: Platelet Activating Factor Acetylhydrolase 2

Gene Aliases: SD-PLA2, Serine-dependent phospholipase A2, Platelet-activating factor acetylhydrolase 2, cytoplasmic

ID gène: 5051

UniProt: Q99487