PVRL1 Kit ELISA

N° du produit ABIN5657967

Détail du produit PVRL1 Kit ELISA

Antigène: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Poliovirus Receptor Related Protein 1 (PVRL1). No significant cross-reactivity or interference between Poliovirus Receptor Related Protein 1 (PVRL1) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poliovirus Receptor Related Protein 1 (PVRL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poliovirus Receptor Related Protein 1 (PVRL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poliovirus Receptor Related Protein 1 (PVRL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poliovirus Receptor Related Protein 1 (PVRL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poliovirus Receptor Related Protein 1 (PVRL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poliovirus Receptor Related Protein 1 (PVRL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Autre désignation: Poliovirus Receptor Related Protein 1 (PVRL1 Produits)

Synonymes: CD111 Kit ELISA, CLPED1 Kit ELISA, ED4 Kit ELISA, HIgR Kit ELISA, HVEC Kit ELISA, OFC7 Kit ELISA, PRR Kit ELISA, PRR1 Kit ELISA, PVRR Kit ELISA, PVRR1 Kit ELISA, SK-12 Kit ELISA, nectin-1 Kit ELISA, prr1 Kit ELISA, PVRL1 Kit ELISA, pvrl1 Kit ELISA, AI835281 Kit ELISA, AW549174 Kit ELISA, Cd111 Kit ELISA, HveC Kit ELISA, cd111 Kit ELISA, cd112 Kit ELISA, clped1 Kit ELISA, ed4 Kit ELISA, higr Kit ELISA, hveb Kit ELISA, hvec Kit ELISA, nectin-2 Kit ELISA, ofc7 Kit ELISA, prr Kit ELISA, prr2 Kit ELISA, pvrl2 Kit ELISA, pvrl2.L Kit ELISA, pvrr Kit ELISA, pvrr1 Kit ELISA, pvrr2 Kit ELISA, sk-12 Kit ELISA, nectin cell adhesion molecule 1 Kit ELISA, nectin-1 Kit ELISA, Poliovirus receptor-related protein 1 Kit ELISA, poliovirus receptor-related 1 (herpesvirus entry mediator C) Kit ELISA, nectin cell adhesion molecule 2 L homeolog Kit ELISA, NECTIN1 Kit ELISA, LOC100145553 Kit ELISA, pvrl1 Kit ELISA, nectin1 Kit ELISA, Nectin1 Kit ELISA, PVRL1 Kit ELISA, nectin2.L Kit ELISA

Sujet:

Gene Name: Poliovirus Receptor Related Protein 1

Gene Aliases: CD111, HVEC, PRR1, CLPED1, ED4, HIgR, OFC7, PRR, PVRR1, SK12, Nectin 1, Herpesvirus Entry Mediator C, Herpesvirus Ig-like receptor

Pathways: Cell-Cell Junction Organization