PABPC1L Kit ELISA

N° du produit ABIN5657971

Détail du produit PABPC1L Kit ELISA

Antigène: PABPC1L (Poly(A) Binding Protein, Cytoplasmic 1-Like (PABPC1L))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L). No significant cross-reactivity or interference between Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L) and analogues was observed.

Sensibilité: 0.115 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PABPC1L (Poly(A) Binding Protein, Cytoplasmic 1-Like (PABPC1L))

Autre désignation: Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L Produits)

Synonymes: fi20g03 Kit ELISA, pab-2 Kit ELISA, wu:fi20g03 Kit ELISA, zgc:55855 Kit ELISA, C20orf119 Kit ELISA, PABPC1L1 Kit ELISA, dJ1069P2.3 Kit ELISA, ePAB Kit ELISA, epabp Kit ELISA, epabp-a Kit ELISA, pabpc1l1 Kit ELISA, 1810053B01Rik Kit ELISA, Epab Kit ELISA, RGD1563142 Kit ELISA, Embryonic poly(A)-binding protein B Kit ELISA, ePAB-B Kit ELISA, ePABP-B Kit ELISA, epab Kit ELISA, epabp-b Kit ELISA, pabpc1l Kit ELISA, pabpc1l-a Kit ELISA, pabpc1l-b Kit ELISA, pabpc1l.S Kit ELISA, poly(A) binding protein, cytoplasmic 1-like Kit ELISA, poly(A) binding protein cytoplasmic 1 like Kit ELISA, poly(A) binding protein, cytoplasmic 1-like S homeolog Kit ELISA, poly(A) binding protein, cytoplasmic 1-like L homeolog Kit ELISA, pabpc1l Kit ELISA, PABPC1L Kit ELISA, pabpc1l.S Kit ELISA, Pabpc1l Kit ELISA, pabpc1l.L Kit ELISA

Sujet:

Gene Name: Poly A Binding Protein Cytoplasmic 1 Like Protein

Gene Aliases: C20orf119, PABPC1L1, ePAB, Polyadenylate-Binding Protein 1-Like

ID gène: 80336

UniProt: Q4VXU2