BMI1 Kit ELISA

N° du produit ABIN5657978

Détail du produit BMI1 Kit ELISA

Antigène: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Polycomb Group Ring Finger Protein 4 (PCGF4). No significant cross-reactivity or interference between Polycomb Group Ring Finger Protein 4 (PCGF4) and analogues was observed.

Sensibilité: 0.122 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polycomb Group Ring Finger Protein 4 (PCGF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polycomb Group Ring Finger Protein 4 (PCGF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polycomb Group Ring Finger Protein 4 (PCGF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polycomb Group Ring Finger Protein 4 (PCGF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polycomb Group Ring Finger Protein 4 (PCGF4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polycomb Group Ring Finger Protein 4 (PCGF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Autre désignation: Polycomb Group Ring Finger Protein 4 (BMI1 Produits)

Synonymes: FLVI2/BMI1 Kit ELISA, PCGF4 Kit ELISA, RNF51 Kit ELISA, AW546694 Kit ELISA, Bmi-1 Kit ELISA, Pcgf4 Kit ELISA, bmi1 Kit ELISA, pcgf4 Kit ELISA, psc1 Kit ELISA, wu:fb17g03 Kit ELISA, wu:fd18f06 Kit ELISA, BMI-1 Kit ELISA, pcgf4b Kit ELISA, bmi-1 Kit ELISA, bmi1-a Kit ELISA, bmi1-b Kit ELISA, bmi1b Kit ELISA, rnf51 Kit ELISA, xbmi-1 Kit ELISA, BMI1 proto-oncogene, polycomb ring finger Kit ELISA, BMI1 polycomb ring finger oncogene Kit ELISA, Bmi1 polycomb ring finger oncogene Kit ELISA, bmi1 polycomb ring finger oncogene 1a Kit ELISA, bmi1 polycomb ring finger oncogene 1b Kit ELISA, BMI1 proto-oncogene, polycomb ring finger L homeolog Kit ELISA, BMI1 Kit ELISA, LOC100230513 Kit ELISA, Bmi1 Kit ELISA, bmi1a Kit ELISA, bmi1b Kit ELISA, bmi1.L Kit ELISA

Sujet:

Gene Name: Polycomb Group Ring Finger Protein 4

Gene Aliases: BMI1, RNF51, BMI1 Polycomb Ring Finger Oncogene, RING finger protein 51, Polycomb complex protein BMI-1

ID gène: 100532731

UniProt: P35226

Pathways: Cycle Cellulaire, Autophagy