PRNP Kit ELISA

N° du produit ABIN5658014

Détail du produit PRNP Kit ELISA

Antigène: PRNP (Prion Protein (PRNP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Prion Protein (PRNP). No significant cross-reactivity or interference between Prion Protein (PRNP) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prion Protein (PRNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prion Protein (PRNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prion Protein (PRNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prion Protein (PRNP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prion Protein (PRNP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prion Protein (PRNP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PRNP (Prion Protein (PRNP))

Autre désignation: Prion Protein (PRNP Produits)

Synonymes: ASCR Kit ELISA, AltPrP Kit ELISA, CD230 Kit ELISA, CJD Kit ELISA, GSS Kit ELISA, KURU Kit ELISA, PRIP Kit ELISA, PrP Kit ELISA, PrP27-30 Kit ELISA, PrP33-35C Kit ELISA, PrPc Kit ELISA, p27-30 Kit ELISA, Prn Kit ELISA, Prn-p Kit ELISA, prp Kit ELISA, cjd Kit ELISA, prion Kit ELISA, prp(c) Kit ELISA, MGC84114 Kit ELISA, PRP Kit ELISA, PRPC Kit ELISA, Prp Kit ELISA, SIP Kit ELISA, PRNP Kit ELISA, AA960666 Kit ELISA, AI325101 Kit ELISA, PrP Kit ELISA, PrPC Kit ELISA, PrPSc Kit ELISA, Prn-i Kit ELISA, Sinc Kit ELISA, prP27-30 Kit ELISA, prP33-35C Kit ELISA, PrP-1 Kit ELISA, PrP1 Kit ELISA, prnprs1 Kit ELISA, stPrP-1 Kit ELISA, zgc:123004 Kit ELISA, prion protein Kit ELISA, prion protein S homeolog Kit ELISA, prion protein b Kit ELISA, PRNP Kit ELISA, Prnp Kit ELISA, prnp.S Kit ELISA, prnpb Kit ELISA

Sujet:

Gene Name: Prion Protein

Gene Aliases: CD230, GSS, ASCR, CJD, PRIP, PrP, PrP2730, PrPc, Major prion protein, Creutzfeldt-Jakob Disease, Gerstmann-Strausler-Scheinker Syndrome, Fatal Familial Insomnia

ID gène: 5621

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation