PIBF1 Kit ELISA

N° du produit ABIN5658068

Détail du produit PIBF1 Kit ELISA

Antigène: PIBF1 (Progesterone Immunomodulatory Binding Factor 1 (PIBF1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Progesterone Immunomodulatory Binding Factor 1 (PIBF1). No significant cross-reactivity or interference between Progesterone Immunomodulatory Binding Factor 1 (PIBF1) and analogues was observed.

Sensibilité: 0.116 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Progesterone Immunomodulatory Binding Factor 1 (PIBF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Progesterone Immunomodulatory Binding Factor 1 (PIBF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Progesterone Immunomodulatory Binding Factor 1 (PIBF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Progesterone Immunomodulatory Binding Factor 1 (PIBF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Progesterone Immunomodulatory Binding Factor 1 (PIBF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Progesterone Immunomodulatory Binding Factor 1 (PIBF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PIBF1 (Progesterone Immunomodulatory Binding Factor 1 (PIBF1))

Autre désignation: Progesterone Immunomodulatory Binding Factor 1 (PIBF1 Produits)

Synonymes: D14Ertd581e Kit ELISA, 1700017E21Rik Kit ELISA, 4930513H15Rik Kit ELISA, 4933438D16Rik Kit ELISA, 4933439E17Rik Kit ELISA, RGD1305077 Kit ELISA, PIBF Kit ELISA, C13orf24 Kit ELISA, zgc:101063 Kit ELISA, MGC80410 Kit ELISA, CEP90 Kit ELISA, RP11-505F3.1 Kit ELISA, progesterone immunomodulatory binding factor 1 Kit ELISA, progesterone immunomodulatory binding factor 1 S homeolog Kit ELISA, Pibf1 Kit ELISA, PIBF1 Kit ELISA, pibf1 Kit ELISA, pibf1.S Kit ELISA

Sujet:

Gene Name: Progesterone Immunomodulatory Binding Factor 1

Gene Aliases: PIBF, C13orf24, CEP90, Progesterone-Induced Blocking Factor 1

ID gène: 10464

UniProt: Q8WXW3