PDCD6 Kit ELISA

N° du produit ABIN5658083

Détail du produit PDCD6 Kit ELISA

Antigène: PDCD6 (Programmed Cell Death 6 (PDCD6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Programmed Cell Death Protein 6 (PDCD6). No significant cross-reactivity or interference between Programmed Cell Death Protein 6 (PDCD6) and analogues was observed.

Sensibilité: 0.118 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 6 (PDCD6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 6 (PDCD6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 6 (PDCD6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Programmed Cell Death Protein 6 (PDCD6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 6 (PDCD6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 6 (PDCD6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PDCD6 (Programmed Cell Death 6 (PDCD6))

Autre désignation: Programmed Cell Death Protein 6 (PDCD6 Produits)

Synonymes: pdcd6 Kit ELISA, MGC52821 Kit ELISA, MGC132114 Kit ELISA, zgc:63952 Kit ELISA, PDCD6 Kit ELISA, ALG-2 Kit ELISA, PEF1B Kit ELISA, AV299538 Kit ELISA, Alg2 Kit ELISA, MA-3 Kit ELISA, PS2 Kit ELISA, alg-2 Kit ELISA, programmed cell death 6 L homeolog Kit ELISA, programmed cell death 6 Kit ELISA, pdcd6.L Kit ELISA, pdcd6 Kit ELISA, PDCD6 Kit ELISA, Pdcd6 Kit ELISA

Sujet:

Gene Name: Programmed Cell Death Protein 6

Gene Aliases: ALG-2, PEF1B, Apoptosis-Linked Gene-2, Probable calcium-binding protein ALG-2

ID gène: 10016

UniProt: O75340

Pathways: Positive Regulation of Endopeptidase Activity