ALIX Kit ELISA

N° du produit ABIN5658084

Détail du produit ALIX Kit ELISA

Antigène: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP). No significant cross-reactivity or interference between Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) and analogues was observed.

Sensibilité: 0.115 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Autre désignation: Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP Produits)

Synonymes: DDBDRAFT_0167145 Kit ELISA, DDBDRAFT_0185177 Kit ELISA, DDB_0167145 Kit ELISA, DDB_0185177 Kit ELISA, AIP1 Kit ELISA, ALIX Kit ELISA, DRIP4 Kit ELISA, HP95 Kit ELISA, AI480591 Kit ELISA, AW544830 Kit ELISA, Aip1 Kit ELISA, Alix Kit ELISA, C76364 Kit ELISA, Eig2 Kit ELISA, mKIAA1375 Kit ELISA, CG12876 Kit ELISA, DAIP1 Kit ELISA, Dmel\\CG12876 Kit ELISA, GB11744 Kit ELISA, RGD1561176 Kit ELISA, zgc:63638 Kit ELISA, wu:fb70d03 Kit ELISA, wu:fy65h02 Kit ELISA, PDCD6IP Kit ELISA, aip1 Kit ELISA, alix Kit ELISA, hp95 Kit ELISA, drip4 Kit ELISA, ALG-2 interacting protein X Kit ELISA, programmed cell death 6 interacting protein Kit ELISA, programmed cell death 6-interacting protein Kit ELISA, Programmed cell death 6 interacting protein Kit ELISA, programmed cell death 6 interacting protein L homeolog Kit ELISA, alxA Kit ELISA, PDCD6IP Kit ELISA, Pdcd6ip Kit ELISA, ALiX Kit ELISA, LOC412662 Kit ELISA, pdcd6ip Kit ELISA, Bm1_48390 Kit ELISA, pdcd6ip.L Kit ELISA

Sujet:

Gene Name: Programmed Cell Death Protein 6 Interacting Protein

Gene Aliases: PDCD6-IP, AIP1, Alix, DRIP4, HP95, ALG-2 Interacting Protein X

ID gène: 10015

UniProt: Q8WUM4

Pathways: Signalisation p53, EGFR Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Tube Formation