PSMA5 Kit ELISA

N° du produit ABIN5658182

Détail du produit PSMA5 Kit ELISA

Antigène: PSMA5 (Proteasome Subunit alpha 5 (PSMA5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Proteasome Subunit Alpha Type 5 (PSMa5). No significant cross-reactivity or interference between Proteasome Subunit Alpha Type 5 (PSMa5) and analogues was observed.

Sensibilité: 0.124 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Subunit Alpha Type 5 (PSMa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteasome Subunit Alpha Type 5 (PSMa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Subunit Alpha Type 5 (PSMa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteasome Subunit Alpha Type 5 (PSMa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteasome Subunit Alpha Type 5 (PSMa5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteasome Subunit Alpha Type 5 (PSMa5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PSMA5 (Proteasome Subunit alpha 5 (PSMA5))

Autre désignation: Proteasome Subunit Alpha Type 5 (PSMA5 Produits)

Synonymes: 154644_at Kit ELISA, Alpha-5 Kit ELISA, CG10938 Kit ELISA, Dmel\\CG10938 Kit ELISA, PRCZ Kit ELISA, PSA5_DROME Kit ELISA, PSMA5 Kit ELISA, ProsMA5 Kit ELISA, alpha5 Kit ELISA, alpha5_dm Kit ELISA, prosMA5 Kit ELISA, DDBDRAFT_0216562 Kit ELISA, DDBDRAFT_0232930 Kit ELISA, DDB_0216562 Kit ELISA, DDB_0232930 Kit ELISA, ZETA Kit ELISA, PSC5 Kit ELISA, zgc:86851 Kit ELISA, Proteasome alpha5 subunit Kit ELISA, proteasome subunit alpha type 5, putative Kit ELISA, proteasome subunit alpha type 5 Kit ELISA, 20S proteasome subunit alpha-5 Kit ELISA, proteasome (prosome, macropain) subunit, alpha type 5 Kit ELISA, proteasome subunit alpha 5 Kit ELISA, proteasome subunit alpha 5 S homeolog Kit ELISA, proteasome (prosome, macropain) subunit, alpha type, 5 Kit ELISA, Prosalpha5 Kit ELISA, PF07_0112 Kit ELISA, CNH01360 Kit ELISA, cgd5_1820 Kit ELISA, PSA5 Kit ELISA, psmA5 Kit ELISA, NAEGRDRAFT_55551 Kit ELISA, LOC100282220 Kit ELISA, LOC100283546 Kit ELISA, Psma5 Kit ELISA, PSMA5 Kit ELISA, psma5.S Kit ELISA, psma5 Kit ELISA

Sujet:

Gene Name: Proteasome Subunit Alpha Type 5

Gene Aliases: PSC5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain

ID gène: 5686

UniProt: P28066

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA