PRKAB1 Kit ELISA

N° du produit ABIN5658232

Détail du produit PRKAB1 Kit ELISA

Antigène: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protein Kinase, AMP Activated Beta 1 (PRKAb1). No significant cross-reactivity or interference between Protein Kinase, AMP Activated Beta 1 (PRKAb1) and analogues was observed.

Sensibilité: 0.124 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Beta 1 (PRKAb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, AMP Activated Beta 1 (PRKAb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, AMP Activated Beta 1 (PRKAb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, AMP Activated Beta 1 (PRKAb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Autre désignation: Protein Kinase, AMP Activated Beta 1 (PRKAB1 Produits)

Synonymes: AMPK Kit ELISA, HAMPKb Kit ELISA, 1300015D22Rik Kit ELISA, AU021155 Kit ELISA, E430008F22 Kit ELISA, MGC82489 Kit ELISA, prkab1 Kit ELISA, wu:fk93d05 Kit ELISA, wu:fw87e09 Kit ELISA, zgc:56652 Kit ELISA, zgc:76975 Kit ELISA, zgc:92228 Kit ELISA, protein kinase AMP-activated non-catalytic subunit beta 1 Kit ELISA, protein kinase, AMP-activated, beta 1 non-catalytic subunit Kit ELISA, protein kinase, AMP-activated, beta 1 non-catalytic subunit S homeolog Kit ELISA, protein kinase, AMP-activated, beta 1 non-catalytic subunit, b Kit ELISA, protein kinase, AMP-activated, beta 1 non-catalytic subunit, a Kit ELISA, PRKAB1 Kit ELISA, Prkab1 Kit ELISA, prkab1.S Kit ELISA, prkab1 Kit ELISA, prkab1b Kit ELISA, prkab1a Kit ELISA

Sujet:

Gene Name: Protein Kinase, AMP Activated Beta 1

Gene Aliases: AMPK, HAMPKb, 5'-AMP-Activated Protein Kinase Subunit Beta-1 Non-Catalytic Subunit, Adenosine Monophosphate Activated Protein Kinase(AMPK)

ID gène: 5564

UniProt: Q9Y478

Pathways: AMPK Signaling, L'effet Warburg