SHP1 Kit ELISA

N° du produit ABIN5658263

Détail du produit SHP1 Kit ELISA

Antigène: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6). No significant cross-reactivity or interference between Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) and analogues was observed.

Sensibilité: 0.105 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Autre désignation: Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6 Produits)

Synonymes: HCPH Kit ELISA, HPTP1C Kit ELISA, PTP-1C Kit ELISA, SH-PTP1 Kit ELISA, SHP-1 Kit ELISA, SHP-1L Kit ELISA, SHP1 Kit ELISA, DRPLA Kit ELISA, Hcph Kit ELISA, Ptp1C Kit ELISA, me Kit ELISA, motheaten Kit ELISA, Ptph6 Kit ELISA, Shp-1 Kit ELISA, fj22f05 Kit ELISA, wu:fj22f05 Kit ELISA, zgc:55501 Kit ELISA, ptpn6 Kit ELISA, protein tyrosine phosphatase, non-receptor type 6 Kit ELISA, protein tyrosine phosphatase, non-receptor type 6 L homeolog Kit ELISA, PTPN6 Kit ELISA, Ptpn6 Kit ELISA, ptpn6 Kit ELISA, ptpn6.L Kit ELISA

Sujet:

Gene Name: Protein Tyrosine Phosphatase, Non Receptor Type 6

Gene Aliases: HCP, HCPH, HPTP1C, SH-PTP1, SHP-1L, SHP1, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1

ID gène: 5777

UniProt: P29350

Pathways: Signalistation JAK/STAT, TCR Signaling, Signalisation TLR, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling