MPZ Kit ELISA

N° du produit ABIN5658270

Détail du produit MPZ Kit ELISA

Antigène: MPZ (Myelin Protein Zero (MPZ))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protein Zero, Myelin (MPZ). No significant cross-reactivity or interference between Protein Zero, Myelin (MPZ) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Zero, Myelin (MPZ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Zero, Myelin (MPZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Zero, Myelin (MPZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Zero, Myelin (MPZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Zero, Myelin (MPZ) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Zero, Myelin (MPZ) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MPZ (Myelin Protein Zero (MPZ))

Autre désignation: Protein Zero, Myelin (MPZ Produits)

Synonymes: CHM Kit ELISA, CMT1 Kit ELISA, CMT1B Kit ELISA, CMT2I Kit ELISA, CMT2J Kit ELISA, CMT4E Kit ELISA, CMTDI3 Kit ELISA, CMTDID Kit ELISA, DSS Kit ELISA, HMSNIB Kit ELISA, MPP Kit ELISA, P0 Kit ELISA, Mpp Kit ELISA, P-zero Kit ELISA, p0 Kit ELISA, sc:d0186 Kit ELISA, wu:fc04b11 Kit ELISA, wu:fi30g06 Kit ELISA, zgc:103775 Kit ELISA, myelin protein zero Kit ELISA, myelin protein zero S homeolog Kit ELISA, MPZ Kit ELISA, Mpz Kit ELISA, mpz Kit ELISA, mpz.S Kit ELISA

Sujet:

Gene Name: Protein Zero, Myelin

Gene Aliases: P0, DSS, CHM, CMT4E, CMT1, CMT1B, CMT2I, CMT2J, CMTDI3, HMSNIB, MPP, Myelin Protein Zero, Charcot-Marie-Tooth Neuropathy 1B, Myelin peripheral protein

ID gène: 4359

UniProt: P25189