PPOX Kit ELISA

N° du produit ABIN5658296

Détail du produit PPOX Kit ELISA

Antigène: PPOX (Protoporphyrinogen Oxidase (PPOX))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protoporphyrinogen Oxidase (PPOX). No significant cross-reactivity or interference between Protoporphyrinogen Oxidase (PPOX) and analogues was observed.

Sensibilité: 0.053 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protoporphyrinogen Oxidase (PPOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protoporphyrinogen Oxidase (PPOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protoporphyrinogen Oxidase (PPOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protoporphyrinogen Oxidase (PPOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protoporphyrinogen Oxidase (PPOX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protoporphyrinogen Oxidase (PPOX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PPOX (Protoporphyrinogen Oxidase (PPOX))

Autre désignation: Protoporphyrinogen Oxidase (PPOX Produits)

Synonymes: PPO Kit ELISA, V290M Kit ELISA, VP Kit ELISA, Ppo Kit ELISA, BcDNA:LD41392 Kit ELISA, CG5796 Kit ELISA, Dmel\\CG5796 Kit ELISA, ESTS:9F2T Kit ELISA, zgc:123252 Kit ELISA, HEMG1 Kit ELISA, PPO1 Kit ELISA, PROTOPORPHYRINOGEN OXIDASE Kit ELISA, TC0121 Kit ELISA, hemG Kit ELISA, protoporphyrinogen oxidase Kit ELISA, Protoporphyrinogen oxidase Kit ELISA, Flavin containing amine oxidoreductase family Kit ELISA, polyphenol oxidase, chloroplastic Kit ELISA, PPOX Kit ELISA, Ppox Kit ELISA, ppox Kit ELISA, hemY Kit ELISA, TC_RS00645 Kit ELISA, Mrub_1406 Kit ELISA, Mesil_2992 Kit ELISA, Trad_0583 Kit ELISA, Calni_0746 Kit ELISA, Ocepr_1049 Kit ELISA, Tmar_0766 Kit ELISA, Intca_1907 Kit ELISA, Despr_0642 Kit ELISA, Plabr_2407 Kit ELISA, Deipr_2130 Kit ELISA, Hipma_0622 Kit ELISA, LOC110782963 Kit ELISA

Sujet:

Gene Name: Protoporphyrinogen Oxidase

Gene Aliases: VP, PPO, V290M, Variegate Porphyria

ID gène: 5498

UniProt: P50336

Pathways: Synaptic Membrane, Feeding Behaviour