NPEPPS Kit ELISA

N° du produit ABIN5658306

Détail du produit NPEPPS Kit ELISA

Antigène: NPEPPS (Aminopeptidase Puromycin Sensitive (NPEPPS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Puromycin Sensitive Aminopeptidase (PSA). No significant cross-reactivity or interference between Puromycin Sensitive Aminopeptidase (PSA) and analogues was observed.

Sensibilité: 0.118 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Puromycin Sensitive Aminopeptidase (PSA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Puromycin Sensitive Aminopeptidase (PSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Puromycin Sensitive Aminopeptidase (PSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Puromycin Sensitive Aminopeptidase (PSA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Puromycin Sensitive Aminopeptidase (PSA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Puromycin Sensitive Aminopeptidase (PSA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NPEPPS (Aminopeptidase Puromycin Sensitive (NPEPPS))

Autre désignation: Puromycin Sensitive Aminopeptidase (NPEPPS Produits)

Synonymes: APS Kit ELISA, KLK2A1 Kit ELISA, PSA Kit ELISA, hK3 Kit ELISA, AAP-S Kit ELISA, MP100 Kit ELISA, CG1009 Kit ELISA, Dmel\\CG1009 Kit ELISA, dPSA Kit ELISA, dPsa Kit ELISA, l(3)06226 Kit ELISA, GB13772 Kit ELISA, Psa Kit ELISA, R74825 Kit ELISA, goku Kit ELISA, psa Kit ELISA, fb68d07 Kit ELISA, sb:cb848 Kit ELISA, wu:fb68d07 Kit ELISA, NPEPPS Kit ELISA, npepps Kit ELISA, MGC147526 Kit ELISA, kallikrein related peptidase 3 Kit ELISA, aminopeptidase puromycin sensitive Kit ELISA, Puromycin sensitive aminopeptidase Kit ELISA, puromycin-sensitive aminopeptidase Kit ELISA, aminopeptidase puromycin sensitive L homeolog Kit ELISA, KLK3 Kit ELISA, NPEPPS Kit ELISA, Psa Kit ELISA, LOC410769 Kit ELISA, Npepps Kit ELISA, npepps Kit ELISA, npepps.L Kit ELISA

Sujet:

Gene Name: Puromycin Sensitive Aminopeptidase

Gene Aliases: NPEPPS, AAP-S, Metalloproteinase MP100, Cytosol alanyl aminopeptidase

ID gène: 9520

UniProt: P55786