PC Kit ELISA

N° du produit ABIN5658309

Détail du produit PC Kit ELISA

Antigène: PC (Pyruvate Carboxylase (PC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Pyruvate Carboxylase (PC). No significant cross-reactivity or interference between Pyruvate Carboxylase (PC) and analogues was observed.

Sensibilité: 0.63 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Carboxylase (PC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Carboxylase (PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Carboxylase (PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Carboxylase (PC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Carboxylase (PC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Carboxylase (PC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PC (Pyruvate Carboxylase (PC))

Autre désignation: Pyruvate Carboxylase (PC Produits)

Synonymes: BcDNA Kit ELISA, BcDNA:GH06348 Kit ELISA, Dmel\\CG1516 Kit ELISA, PC Kit ELISA, pyc Kit ELISA, wu:fb82g08 Kit ELISA, wu:fj85g01 Kit ELISA, PCX Kit ELISA, BA4157 Kit ELISA, Pc Kit ELISA, Pcb Kit ELISA, PCB Kit ELISA, Pcx Kit ELISA, Pyruvate carboxylase Kit ELISA, pyruvate carboxylase b Kit ELISA, PYruvate Carboxylase Kit ELISA, pyruvate carboxylase Kit ELISA, pyruvate carboxylase Pyr1 Kit ELISA, PCB Kit ELISA, pcxb Kit ELISA, pyc-1 Kit ELISA, PC Kit ELISA, pyc Kit ELISA, pyr1 Kit ELISA, CNF00650 Kit ELISA, PYC1 Kit ELISA, Ndas_1152 Kit ELISA, Ilyop_2503 Kit ELISA, Calni_0495 Kit ELISA, Ftrac_1004 Kit ELISA, Plabr_3574 Kit ELISA, Sgly_2936 Kit ELISA, Celly_0558 Kit ELISA, Hipma_0131 Kit ELISA, Halhy_1567 Kit ELISA, Mesop_1269 Kit ELISA, Pcx Kit ELISA, Pc Kit ELISA

Sujet:

Gene Name: Pyruvate Carboxylase

Gene Aliases: PCB

ID gène: 5091

UniProt: P11498