PDHB Kit ELISA

N° du produit ABIN5658315

Détail du produit PDHB Kit ELISA

Antigène: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Beta (PDHb). No significant cross-reactivity or interference between Pyruvate Dehydrogenase Beta (PDHb) and analogues was observed.

Sensibilité: 0.066 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Beta (PDHb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Beta (PDHb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Beta (PDHb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Beta (PDHb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Dehydrogenase Beta (PDHb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Dehydrogenase Beta (PDHb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Autre désignation: Pyruvate Dehydrogenase Beta (PDHB Produits)

Synonymes: CG 11876 Kit ELISA, Dmel\\CG11876 Kit ELISA, E1beta Kit ELISA, PDHB Kit ELISA, zgc:64062 Kit ELISA, wu:fc76a05 Kit ELISA, PDHBD Kit ELISA, PDHE1-B Kit ELISA, PHE1B Kit ELISA, 2610103L06Rik Kit ELISA, AL024199 Kit ELISA, C81408 Kit ELISA, CG11876 gene product from transcript CG11876-RA Kit ELISA, pyruvate dehydrogenase E1 beta subunit Kit ELISA, pyruvate dehydrogenase (lipoamide) beta Kit ELISA, CG11876 Kit ELISA, pdhb Kit ELISA, PDHB Kit ELISA, Pdhb Kit ELISA

Sujet:

Gene Name: Pyruvate Dehydrogenase Beta

Gene Aliases: PDH-B, PHE1B, PHE1-B, Pyruvate Dehydrogenase(lipoamide)Beta

ID gène: 68263

UniProt: Q9D051

Pathways: L'effet Warburg