PKM Kit ELISA

N° du produit ABIN5658324

Détail du produit PKM Kit ELISA

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.94 ng/mL - 60 ng/mL

Seuil minimal de détection: 0.94 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Pyruvate Kinase, Muscle (PKM2). No significant cross-reactivity or interference between Pyruvate Kinase, Muscle (PKM2) and analogues was observed.

Sensibilité: 0.42 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Muscle (PKM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Kinase, Muscle (PKM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Kinase, Muscle (PKM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Kinase, Muscle (PKM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Kinase, Muscle (PKM2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Kinase, Muscle (PKM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Autre désignation: Pyruvate Kinase, Muscle (PKM Produits)

Synonymes: CTHBP Kit ELISA, OIP3 Kit ELISA, PK3 Kit ELISA, PKM2 Kit ELISA, TCB Kit ELISA, THBP1 Kit ELISA, AA414905 Kit ELISA, AL024370 Kit ELISA, AL024424 Kit ELISA, Pk-2 Kit ELISA, Pk-3 Kit ELISA, Pk3 Kit ELISA, Pkm2 Kit ELISA, PKM12 Kit ELISA, CG7070 Kit ELISA, Dmel\\CG7070 Kit ELISA, PYK Kit ELISA, Pyk Kit ELISA, cb1029 Kit ELISA, fj33f03 Kit ELISA, pkm2 Kit ELISA, pkm2a Kit ELISA, wu:fj33f03 Kit ELISA, MGC53388 Kit ELISA, PKM Kit ELISA, PK-S Kit ELISA, pk3 Kit ELISA, pkm2b Kit ELISA, zgc:92037 Kit ELISA, pyruvate kinase M1/2 Kit ELISA, pyruvate kinase, muscle Kit ELISA, Pyruvate kinase Kit ELISA, pyruvate kinase M1/2a Kit ELISA, pyruvate kinase PKM Kit ELISA, pyruvate kinase (PK) Kit ELISA, pyruvate kinase Kit ELISA, pyruvate kinase M1/2 S homeolog Kit ELISA, pyruvate kinase M1/2b Kit ELISA, PKM Kit ELISA, Pkm Kit ELISA, PyK Kit ELISA, pkma Kit ELISA, pkm Kit ELISA, LOC710798 Kit ELISA, pyk Kit ELISA, pk Kit ELISA, LOC100534543 Kit ELISA, pkm.S Kit ELISA, pkmb Kit ELISA

Sujet:

Gene Name: Pyruvate Kinase, Muscle

Gene Aliases: M2PK, PKM, M2-PK, PKM1, CTHBP, OIP3, PK3, PKM, TCB, THBP1, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Cytosolic thyroid hormone-binding protein

ID gène: 25630

UniProt: P11980

Pathways: L'effet Warburg