RAB5 Kit CLIA

N° du produit ABIN5658340

Détail du produit RAB5 Kit CLIA

Antigène: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 0.594 ng/mL - 38 ng/mL

Seuil minimal de détection: 0.594 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of RAB5A, Member RAS Oncogene Family (RAB5A). No significant cross-reactivity or interference between RAB5A, Member RAS Oncogene Family (RAB5A) and analogues was observed.

Sensibilité: 0.02 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the RAB5A, Member RAS Oncogene Family (RAB5A) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Autre désignation: RAB5A, Member RAS Oncogene Family (RAB5A Produits)

Synonymes: RAB5 Kit CLIA, 2410015H04Rik Kit CLIA, AI663973 Kit CLIA, AU021172 Kit CLIA, nnyRab5a Kit CLIA, DDBDRAFT_0168594 Kit CLIA, DDBDRAFT_0229401 Kit CLIA, DDB_0168594 Kit CLIA, DDB_0229401 Kit CLIA, rab5 Kit CLIA, RAB5A Kit CLIA, Rab5a Kit CLIA, rab5al Kit CLIA, zgc:56644 Kit CLIA, fj38g08 Kit CLIA, hm:zehn1144 Kit CLIA, rab5a Kit CLIA, wu:fj38g08 Kit CLIA, RAB5A, member RAS oncogene family Kit CLIA, rab5A protein Kit CLIA, Rab5a, GTPase Kit CLIA, predicted protein Kit CLIA, Rab GTPase Kit CLIA, RAB5A, member RAS oncogene family L homeolog Kit CLIA, RAB5A, member RAS oncogene family, b Kit CLIA, RAB5A, member RAS oncogene family, a Kit CLIA, RAB5A Kit CLIA, Rab5a Kit CLIA, rab5A Kit CLIA, rab5a Kit CLIA, rab5a.L Kit CLIA, rab5ab Kit CLIA, rab5aa Kit CLIA

Sujet:

Gene Name: RAB5A, Member RAS Oncogene Family

Gene Aliases: RAB5

ID gène: 5868

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity