RACGAP1 Kit ELISA

N° du produit ABIN5658346

Détail du produit RACGAP1 Kit ELISA

Antigène: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Rac-GTPase Activating Protein 1 (RACGAP1). No significant cross-reactivity or interference between Rac-GTPase Activating Protein 1 (RACGAP1) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rac-GTPase Activating Protein 1 (RACGAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rac-GTPase Activating Protein 1 (RACGAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rac-GTPase Activating Protein 1 (RACGAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rac-GTPase Activating Protein 1 (RACGAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rac-GTPase Activating Protein 1 (RACGAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rac-GTPase Activating Protein 1 (RACGAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Autre désignation: Rac-GTPase Activating Protein 1 (RACGAP1 Produits)

Synonymes: CYK4 Kit ELISA, HsCYK-4 Kit ELISA, ID-GAP Kit ELISA, MgcRacGAP Kit ELISA, cyk-4 Kit ELISA, id-gap Kit ELISA, mgcracgap Kit ELISA, RACGAP1 Kit ELISA, MGC121578 Kit ELISA, Gap Kit ELISA, RasGAP Kit ELISA, Rasa Kit ELISA, GAPX Kit ELISA, AI227039 Kit ELISA, AI327394 Kit ELISA, Band25 Kit ELISA, GTPase Kit ELISA, gtl11 Kit ELISA, mKIAA1478 Kit ELISA, wu:fb92c08 Kit ELISA, zgc:77839 Kit ELISA, Rac GTPase activating protein 1 Kit ELISA, rac GTPase-activating protein 1 Kit ELISA, Rac GTPase activating protein 1 L homeolog Kit ELISA, RAS p21 protein activator 1 Kit ELISA, Rac GTPase-activating protein 1 Kit ELISA, si:ch1073-416j23.1 Kit ELISA, RACGAP1 Kit ELISA, racgap1 Kit ELISA, racgap1.L Kit ELISA, racgap1.2.L Kit ELISA, racgap1.2 Kit ELISA, Rasa1 Kit ELISA, Racgap1 Kit ELISA, si:ch1073-416j23.1 Kit ELISA

Sujet:

Gene Name: Rac-GTPase Activating Protein 1

Gene Aliases: HsCYK-4, ID-GAP, MgcRacGAP, Male germ cell RacGap

ID gène: 29127

UniProt: Q9H0H5

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor