RAD51 Homolog Kit ELISA

N° du produit ABIN5658348

Détail du produit RAD51 Homolog Kit ELISA

Antigène: RAD51 Homolog (RAD51)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of RAD51 Homolog (RAD51). No significant cross-reactivity or interference between RAD51 Homolog (RAD51) and analogues was observed.

Sensibilité: 0.118 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAD51 Homolog (RAD51). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAD51 Homolog (RAD51). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAD51 Homolog (RAD51), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAD51 Homolog (RAD51) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAD51 Homolog (RAD51) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAD51 Homolog (RAD51) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RAD51 Homolog (RAD51)

Autre désignation: RAD51 Homolog (RAD51 Produits)

Synonymes: BRCC5 Kit ELISA, HRAD51 Kit ELISA, HsRad51 Kit ELISA, HsT16930 Kit ELISA, MRMV2 Kit ELISA, RAD51A Kit ELISA, RECA Kit ELISA, RAD51 recombinase Kit ELISA, RAD51 Kit ELISA

Sujet:

Gene Name: RAD51 Homolog

Gene Aliases: RAD51A, RECA, HsRad51, BRCC5, BRCA1/BRCA2-Containing Complex Subunit 5, DNA repair protein RAD51 homolog 1

ID gène: 5888

UniProt: Q06609