ROMO1 Kit ELISA

N° du produit ABIN5658361

Détail du produit ROMO1 Kit ELISA

Antigène: ROMO1 (Reactive Oxygen Species Modulator 1 (ROMO1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Reactive Oxygen Species Modulator 1 (ROMO1). No significant cross-reactivity or interference between Reactive Oxygen Species Modulator 1 (ROMO1) and analogues was observed.

Sensibilité: 0.053 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Reactive Oxygen Species Modulator 1 (ROMO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Reactive Oxygen Species Modulator 1 (ROMO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Reactive Oxygen Species Modulator 1 (ROMO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Reactive Oxygen Species Modulator 1 (ROMO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Reactive Oxygen Species Modulator 1 (ROMO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Reactive Oxygen Species Modulator 1 (ROMO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ROMO1 (Reactive Oxygen Species Modulator 1 (ROMO1))

Autre désignation: Reactive Oxygen Species Modulator 1 (ROMO1 Produits)

Synonymes: romo1 Kit ELISA, zgc:77111 Kit ELISA, C20orf52 Kit ELISA, mtgmp Kit ELISA, RA_r333_jsm669B4f Kit ELISA, MTGM Kit ELISA, MTGMP Kit ELISA, bA353C18.2 Kit ELISA, 2010100O12Rik Kit ELISA, AI853864 Kit ELISA, C13H20ORF52 Kit ELISA, MGR2 Kit ELISA, reactive oxygen species modulator 1 Kit ELISA, reactive oxygen species modulator 1 L homeolog Kit ELISA, romo1 Kit ELISA, ROMO1 Kit ELISA, Romo1 Kit ELISA, romo1.L Kit ELISA

Sujet:

Gene Name: Reactive Oxygen Species Modulator 1

Gene Aliases: C20orf52, MTGMP, Glyrichin, Mitochondrial Targeting GXXXG Protein, Epididymis tissue protein Li 175, Protein MGR2 homolog