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FCGR1 Kit ELISA

FCGR1 Reactivité: Humain Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5658373
  • Antigène Voir toutes FCGR1 Kits ELISA
    FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))
    Reactivité
    • 2
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78 pg/mL - 5000 pg/mL
    Seuil minimal de détection
    78 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). No significant cross-reactivity or interference between Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) and analogues was observed.
    Sensibilité
    31 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor I For The Fc Region Of Immunoglobulin G (FcgRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes FCGR1 Kits ELISA
    FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))
    Autre désignation
    Receptor I For The Fc Region Of Immunoglobulin G (FCGR1 Produits)
    Synonymes
    CD64 Kit ELISA, CD64A Kit ELISA, FCRI Kit ELISA, IGFR1 Kit ELISA, AI323638 Kit ELISA, AV092959 Kit ELISA, FcgammaRI Kit ELISA, IGGHAFC Kit ELISA, FCGR1A Kit ELISA, Fcgr1 Kit ELISA, Fcgr1b Kit ELISA, Fc fragment of IgG receptor Ia Kit ELISA, Fc receptor, IgG, high affinity I Kit ELISA, FCGR1A Kit ELISA, Fcgr1 Kit ELISA, Fcgr1a Kit ELISA
    Sujet

    Gene Name: Receptor I For The Fc Region Of Immunoglobulin G

    Gene Aliases: CD64, FCGR1, FCGR1A, FCGR1B, FCGR1C, FcgRIA, CD64A, FcgRIB, CD64B, FcgRIC, CD64C, High affinity immunoglobulin gamma Fc receptor I

    ID gène
    2209
    UniProt
    P12314
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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