Fc gamma RII (CD32) Kit ELISA

N° du produit ABIN5658377

Détail du produit

Antigène: Fc gamma RII (CD32)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 46.88 pg/mL - 3000 pg/mL

Seuil minimal de détection: 46.88 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Receptor II For The Fc Region Of Immunoglobulin G (FcgRII). No significant cross-reactivity or interference between Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) and analogues was observed.

Sensibilité: 17.35 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor II For The Fc Region Of Immunoglobulin G (FcgRII). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor II For The Fc Region Of Immunoglobulin G (FcgRII). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor II For The Fc Region Of Immunoglobulin G (FcgRII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Fc gamma RII (CD32)

Autre désignation: Receptor II For The Fc Region Of Immunoglobulin G (CD32 Produits)

Synonymes: CD32 Kit ELISA, CD32B Kit ELISA, FCG2 Kit ELISA, FCGR2 Kit ELISA, IGFR2 Kit ELISA, FCGR2B Kit ELISA, AI528646 Kit ELISA, F630109E10Rik Kit ELISA, Fc[g]RII Kit ELISA, FcgRII Kit ELISA, Fcgr2 Kit ELISA, Fcgr2a Kit ELISA, Fcr-2 Kit ELISA, Fcr-3 Kit ELISA, Ly-17 Kit ELISA, Ly-m20 Kit ELISA, LyM-1 Kit ELISA, Lym-1 Kit ELISA, FCRII Kit ELISA, FcRII Kit ELISA, Fc fragment of IgG receptor IIb Kit ELISA, Fc receptor, IgG, low affinity IIb Kit ELISA, FCGR2B Kit ELISA, Fcgr2b Kit ELISA

Classe de substances: Antibody

Sujet:

Gene Name: Receptor II For The Fc Region Of Immunoglobulin G

Gene Aliases: CD32, FCGR2A, FCG2, FCGR2A1, FCGR2, FCGR2B, FCGR2C, Fc Fragment Of IgG, Low Affinity IIa, Receptor, Low affinity immunoglobulin gamma Fc region receptor II-a

ID gène: 2212

UniProt: P12318