RIPK1 Kit ELISA

N° du produit ABIN5658378

Détail du produit RIPK1 Kit ELISA

Antigène: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Receptor Interacting Serine Threonine Kinase 1 (RIPK1). No significant cross-reactivity or interference between Receptor Interacting Serine Threonine Kinase 1 (RIPK1) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 1 (RIPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor Interacting Serine Threonine Kinase 1 (RIPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 1 (RIPK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 1 (RIPK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Autre désignation: Receptor Interacting Serine Threonine Kinase 1 (RIPK1 Produits)

Synonymes: RIP Kit ELISA, RIP1 Kit ELISA, D330015H01Rik Kit ELISA, Rinp Kit ELISA, Rip1 Kit ELISA, rip1 Kit ELISA, ripk1 Kit ELISA, xRIP1beta Kit ELISA, xrip1 Kit ELISA, receptor interacting serine/threonine kinase 1 Kit ELISA, receptor (TNFRSF)-interacting serine-threonine kinase 1 Kit ELISA, receptor interacting serine/threonine kinase 1 L homeolog Kit ELISA, RIPK1 Kit ELISA, Ripk1 Kit ELISA, ripk1.L Kit ELISA

Sujet:

Gene Name: Receptor Interacting Serine Threonine Kinase 1

Gene Aliases: RIP, Receptor-Interacting Protein, Cell death protein RIP, Serine/threonine-protein kinase RIP

ID gène: 8737

UniProt: Q13546

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway