Regucalcin Kit ELISA

N° du produit ABIN5658405

Détail du produit Regucalcin Kit ELISA

Antigène: Regucalcin (RGN)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Regucalcin (RGN). No significant cross-reactivity or interference between Regucalcin (RGN) and analogues was observed.

Sensibilité: 0.109 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regucalcin (RGN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regucalcin (RGN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regucalcin (RGN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regucalcin (RGN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regucalcin (RGN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regucalcin (RGN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Regucalcin (RGN)

Autre désignation: Regucalcin (RGN Produits)

Synonymes: CG1803 Kit ELISA, Dmel\\CG1803 Kit ELISA, Regucalcin Kit ELISA, T1 Kit ELISA, GNL Kit ELISA, zgc:92078 Kit ELISA, smp30 Kit ELISA, xsmp-30 Kit ELISA, xsmp30 Kit ELISA, DyakGE16489 Kit ELISA, dyak_GLEANR_17905 Kit ELISA, GE16489 Kit ELISA, regucalcin Kit ELISA, SMP30 Kit ELISA, AI265316 Kit ELISA, RC Kit ELISA, rgn-A Kit ELISA, Rc Kit ELISA, Reguc Kit ELISA, SMP-30 Kit ELISA, CG1803 gene product from transcript CG1803-RA Kit ELISA, regucalcin Kit ELISA, GE16489 gene product from transcript GE16489-RB Kit ELISA, regucalcin (senescence marker protein-30) Kit ELISA, regucalcin L homeolog Kit ELISA, regucalcin Kit ELISA, rgn Kit ELISA, LOC465598 Kit ELISA, RGN Kit ELISA, Dyak\regucalcin Kit ELISA, Rgn Kit ELISA, rgn.L Kit ELISA

Sujet:

Gene Name: Regucalcin

Gene Aliases: RC, SMP30, GNL, Senescence Marker Protein-30, Gluconolactonase

ID gène: 9104

UniProt: Q15493