RGS5 Kit ELISA

N° du produit ABIN5658418

Détail du produit RGS5 Kit ELISA

Antigène: RGS5 (Regulator of G-Protein Signaling 5 (RGS5))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Regulator Of G Protein Signaling 5 (RGS5). No significant cross-reactivity or interference between Regulator Of G Protein Signaling 5 (RGS5) and analogues was observed.

Sensibilité: 0.067 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulator Of G Protein Signaling 5 (RGS5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulator Of G Protein Signaling 5 (RGS5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulator Of G Protein Signaling 5 (RGS5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regulator Of G Protein Signaling 5 (RGS5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulator Of G Protein Signaling 5 (RGS5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulator Of G Protein Signaling 5 (RGS5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RGS5 (Regulator of G-Protein Signaling 5 (RGS5))

Autre désignation: Regulator Of G Protein Signaling 5 (RGS5 Produits)

Synonymes: MST092 Kit ELISA, MST106 Kit ELISA, MST129 Kit ELISA, MSTP032 Kit ELISA, MSTP092 Kit ELISA, MSTP106 Kit ELISA, MSTP129 Kit ELISA, RGS5 Kit ELISA, 1110070A02Rik Kit ELISA, fj30h05 Kit ELISA, rgs5 Kit ELISA, wu:fj30h05 Kit ELISA, zgc:64006 Kit ELISA, xrgs5 Kit ELISA, MGC147439 Kit ELISA, zgc:110206 Kit ELISA, regulator of G protein signaling 5 Kit ELISA, regulator of G-protein signaling 5 Kit ELISA, regulator of G protein signaling 5a Kit ELISA, regulator of G-protein signaling 5 L homeolog Kit ELISA, regulator of G protein signaling 5b Kit ELISA, RGS5 Kit ELISA, Rgs5 Kit ELISA, rgs5a Kit ELISA, rgs5 Kit ELISA, rgs5.L Kit ELISA, rgs5b Kit ELISA

Sujet:

Gene Name: Regulator Of G Protein Signaling 5

ID gène: 19737

UniProt: O08850

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling