RPA1 Kit ELISA

N° du produit ABIN5658446

Détail du produit RPA1 Kit ELISA

Antigène: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Replication Protein A1 (RPA1). No significant cross-reactivity or interference between Replication Protein A1 (RPA1) and analogues was observed.

Sensibilité: 0.128 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Replication Protein A1 (RPA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Replication Protein A1 (RPA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Replication Protein A1 (RPA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Replication Protein A1 (RPA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Replication Protein A1 (RPA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Replication Protein A1 (RPA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RPA1 (Replication Protein A1, 70kDa (RPA1))

Autre désignation: Replication Protein A1 (RPA1 Produits)

Synonymes: HSSB Kit ELISA, MST075 Kit ELISA, REPA1 Kit ELISA, RF-A Kit ELISA, RP-A Kit ELISA, RPA70 Kit ELISA, Cb1-727 Kit ELISA, wu:fi14b08 Kit ELISA, zgc:55337 Kit ELISA, zgc:77092 Kit ELISA, RPA1 Kit ELISA, hssb Kit ELISA, mst075 Kit ELISA, repa1 Kit ELISA, rf-a Kit ELISA, rp-a Kit ELISA, rpa Kit ELISA, rpa70 Kit ELISA, LOC100230990 Kit ELISA, 5031405K23Rik Kit ELISA, 70kDa Kit ELISA, AA589576 Kit ELISA, AW557552 Kit ELISA, Rpa Kit ELISA, CG9633 Kit ELISA, D-RPA70 Kit ELISA, D-SSB Kit ELISA, DRP-A Kit ELISA, DmRPA Kit ELISA, Dmel\\CG9633 Kit ELISA, RPA Kit ELISA, RPA 70 Kit ELISA, RPA 70 kDa Kit ELISA, RpA70 Kit ELISA, Rpa-70 Kit ELISA, Ssb-70 Kit ELISA, dRP-A Kit ELISA, dmrpa1 Kit ELISA, i164 Kit ELISA, replication protein A1 Kit ELISA, replication protein A1, 70kDa Kit ELISA, replication protein A1 L homeolog Kit ELISA, Replication Protein A 70 Kit ELISA, RPA1 Kit ELISA, Rpa1 Kit ELISA, rpa1 Kit ELISA, rpa1.L Kit ELISA, RpA-70 Kit ELISA

Sujet:

Gene Name: Replication Protein A1

Gene Aliases: HSSB, REPA1, RF-A, RP-A, RPA70, Replication protein A 70 kDa DNA-binding subunit, Single-stranded DNA-binding protein

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA