Retinoic Acid Receptor alpha Kit ELISA

N° du produit ABIN5658465

Détail du produit Retinoic Acid Receptor alpha Kit ELISA

Antigène: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Retinoic Acid Receptor Alpha (RARa). No significant cross-reactivity or interference between Retinoic Acid Receptor Alpha (RARa) and analogues was observed.

Sensibilité: 0.114 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoic Acid Receptor Alpha (RARa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoic Acid Receptor Alpha (RARa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoic Acid Receptor Alpha (RARa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoic Acid Receptor Alpha (RARa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoic Acid Receptor Alpha (RARa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoic Acid Receptor Alpha (RARa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Autre désignation: Retinoic Acid Receptor Alpha (RARA Produits)

Synonymes: NR1B1 Kit ELISA, RAR Kit ELISA, Nr1b1 Kit ELISA, RARalpha1 Kit ELISA, rar-alpha Kit ELISA, etID309833.12 Kit ELISA, rara Kit ELISA, rara2a Kit ELISA, zRAR Kit ELISA, zRAR-alpha Kit ELISA, zgc:109797 Kit ELISA, nr1b1 Kit ELISA, RAR-ALPHA1 Kit ELISA, HS-RARa Kit ELISA, fj66e06 Kit ELISA, rara2b Kit ELISA, wu:fj66e06 Kit ELISA, retinoic acid receptor alpha Kit ELISA, retinoic acid receptor, alpha Kit ELISA, retinoic acid receptor, alpha a Kit ELISA, retinoic acid receptor alpha L homeolog Kit ELISA, retinoic acid receptor, alpha b Kit ELISA, RARA Kit ELISA, Rara Kit ELISA, LOC100136372 Kit ELISA, raraa Kit ELISA, rara.L Kit ELISA, rara Kit ELISA, rarab Kit ELISA

Sujet:

Gene Name: Retinoic Acid Receptor Alpha

Gene Aliases: RAR-A, NR1B1, RAR, RAR-Alpha, Nuclear Receptor Subfamily 1 Group B Member 1

ID gène: 24705

UniProt: Q499N1

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, S100 Proteins