RBP3 Kit ELISA

N° du produit ABIN5658475

Détail du produit RBP3 Kit ELISA

Antigène: RBP3 (Retinol Binding Protein 3, Interstitial (RBP3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Retinol Binding Protein 3, Interstitial (RBP3). No significant cross-reactivity or interference between Retinol Binding Protein 3, Interstitial (RBP3) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinol Binding Protein 3, Interstitial (RBP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinol Binding Protein 3, Interstitial (RBP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinol Binding Protein 3, Interstitial (RBP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinol Binding Protein 3, Interstitial (RBP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinol Binding Protein 3, Interstitial (RBP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinol Binding Protein 3, Interstitial (RBP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RBP3 (Retinol Binding Protein 3, Interstitial (RBP3))

Autre désignation: Retinol Binding Protein 3, Interstitial (RBP3 Produits)

Synonymes: Irbp Kit ELISA, Rbp-3 Kit ELISA, D10S64 Kit ELISA, D10S65 Kit ELISA, D10S66 Kit ELISA, IRBP Kit ELISA, RBPI Kit ELISA, RP66 Kit ELISA, retinol binding protein 3 Kit ELISA, interphotoreceptor retinoid-binding protein Kit ELISA, retinol binding protein 3, interstitial Kit ELISA, rbp3 Kit ELISA, RBP3 Kit ELISA, glr2547 Kit ELISA, XOO2681 Kit ELISA, AMED_0162 Kit ELISA, ambt_08395 Kit ELISA, Rbp3 Kit ELISA

Sujet:

Gene Name: Retinol Binding Protein 3, Interstitial

Gene Aliases: RBP-3, IRBP, RBPI, Interphotoreceptor retinoid-binding protein, Interstitial retinol-binding protein

ID gène: 5949

UniProt: P10745