RHD Kit ELISA

N° du produit ABIN5658483

Détail du produit RHD Kit ELISA

Antigène: RHD (Rh Blood Group, D Antigen (RHD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 2.47 ng/mL - 200 ng/mL

Seuil minimal de détection: 2.47 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Rh Blood Group, D Antigen (RHD). No significant cross-reactivity or interference between Rh Blood Group, D Antigen (RHD) and analogues was observed.

Sensibilité: 0.92 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 h

Plaque: Pre-coated

Protocole: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Rh Blood Group, D Antigen (RHD) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Rh Blood Group, D Antigen (RHD) and unlabeled Rh Blood Group, D Antigen (RHD) (Standards or samples) with the pre-coated antibody specific to Rh Blood Group, D Antigen (RHD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Rh Blood Group, D Antigen (RHD) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Rh Blood Group, D Antigen (RHD) in the sample.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rh Blood Group, D Antigen (RHD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rh Blood Group, D Antigen (RHD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RHD (Rh Blood Group, D Antigen (RHD))

Autre désignation: Rh Blood Group, D Antigen (RHD Produits)

Synonymes: CD240D Kit ELISA, DIIIc Kit ELISA, RH Kit ELISA, RH30 Kit ELISA, RHCED Kit ELISA, RHDVA(TT) Kit ELISA, RHDel Kit ELISA, RHPII Kit ELISA, RHXIII Kit ELISA, Rh4 Kit ELISA, RhDCw Kit ELISA, RhII Kit ELISA, RhK562-II Kit ELISA, RhPI Kit ELISA, Rh Kit ELISA, Rhced Kit ELISA, Rhl1 Kit ELISA, Rh blood group D antigen Kit ELISA, Rh blood group, D antigen Kit ELISA, RHD Kit ELISA, Rhd Kit ELISA

Sujet:

Gene Name: Rh Blood Group, D Antigen

Gene Aliases: CD240D, Rh30a, Rh4, RhPI, RhII, DIIIc, Rhesus D antigen, Rh polypeptide 2

ID gène: 6007

UniProt: Q02161