ROCK2 Kit ELISA

N° du produit ABIN5658484

Détail du produit ROCK2 Kit ELISA

Antigène: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2). No significant cross-reactivity or interference between Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Autre désignation: Rho Associated Coiled Coil Containing Protein Kinase 2 (ROCK2 Produits)

Synonymes: rocka-a Kit ELISA, xROK Kit ELISA, rock2 Kit ELISA, rok2 Kit ELISA, ROCK2 Kit ELISA, B230113H15Rik Kit ELISA, ROKalpha Kit ELISA, Rho-kinase Kit ELISA, Rock-II Kit ELISA, Rock2m Kit ELISA, mKIAA0619 Kit ELISA, ROCK-II Kit ELISA, ROK Kit ELISA, Rho-associated, coiled-coil containing protein kinase 2 L homeolog Kit ELISA, rho-associated, coiled-coil containing protein kinase 2a Kit ELISA, Rho associated coiled-coil containing protein kinase 2 Kit ELISA, Rho-associated, coiled-coil containing protein kinase 2 Kit ELISA, Rho-associated coiled-coil containing protein kinase 2 Kit ELISA, rock2.L Kit ELISA, rock2a Kit ELISA, ROCK2 Kit ELISA, Rock2 Kit ELISA

Sujet:

Gene Name: Rho Associated Coiled Coil Containing Protein Kinase 2

ID gène: 9475

UniProt: O75116

Pathways: Dynamique des Microtubules, Signalisation WNT, Tube Formation