RRM1 Kit ELISA

N° du produit ABIN5658511

Détail du produit RRM1 Kit ELISA

Antigène: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ribonucleotide Reductase M1 (RRM1). No significant cross-reactivity or interference between Ribonucleotide Reductase M1 (RRM1) and analogues was observed.

Sensibilité: 0.115 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonucleotide Reductase M1 (RRM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonucleotide Reductase M1 (RRM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonucleotide Reductase M1 (RRM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonucleotide Reductase M1 (RRM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonucleotide Reductase M1 (RRM1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonucleotide Reductase M1 (RRM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Autre désignation: Ribonucleotide Reductase M1 (RRM1 Produits)

Synonymes: CG5371 Kit ELISA, DmRNR1 Kit ELISA, Dmel\\CG5371 Kit ELISA, RNR1 Kit ELISA, RnrM1 Kit ELISA, RntL Kit ELISA, l(2)k06709 Kit ELISA, XRRM1 Kit ELISA, R1 Kit ELISA, RIR1 Kit ELISA, RR1 Kit ELISA, CHUNP6866 Kit ELISA, cb396 Kit ELISA, cb838 Kit ELISA, r1 Kit ELISA, sb:cb548 Kit ELISA, wu:fb39b07 Kit ELISA, wu:fi14b02 Kit ELISA, wu:fk95f07 Kit ELISA, ribonucleotide reductase catalytic subunit M1 Kit ELISA, Ribonucleoside diphosphate reductase large subunit Kit ELISA, ribonucleoside-diphosphate reductase large subunit Kit ELISA, ribonucleotide reductase M1 L homeolog Kit ELISA, ribonucleotide reductase M1 Kit ELISA, ribonucleotide reductase M1 polypeptide Kit ELISA, RRM1 Kit ELISA, RnrL Kit ELISA, LOC100472317 Kit ELISA, rrm1 Kit ELISA, rrm1.L Kit ELISA, Rrm1 Kit ELISA

Sujet:

Gene Name: Ribonucleotide Reductase M1

Gene Aliases: R1, RIR1, RR1, Ribonucleoside-diphosphate reductase large subunit

ID gène: 6240

UniProt: P23921

Pathways: Positive Regulation of Endopeptidase Activity