RPL13A Kit ELISA

N° du produit ABIN5658514

Détail du produit RPL13A Kit ELISA

Antigène: RPL13A (Ribosomal Protein L13a (RPL13A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein L13A (RPL13A). No significant cross-reactivity or interference between Ribosomal Protein L13A (RPL13A) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein L13A (RPL13A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein L13A (RPL13A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein L13A (RPL13A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein L13A (RPL13A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribosomal Protein L13A (RPL13A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribosomal Protein L13A (RPL13A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RPL13A (Ribosomal Protein L13a (RPL13A))

Autre désignation: Ribosomal Protein L13A (RPL13A Produits)

Synonymes: 1810026N22Rik Kit ELISA, Tstap198-7 Kit ELISA, tum-antigen Kit ELISA, L13A Kit ELISA, TSTA1 Kit ELISA, CG1475 Kit ELISA, Dmel\\CG1475 Kit ELISA, L13a2 Kit ELISA, M(3)83B Kit ELISA, Rp L13A Kit ELISA, anon-EST:Posey125 Kit ELISA, anon-EST:fe1A5 Kit ELISA, bs25f04.y1 Kit ELISA, cg1475 Kit ELISA, MGC54018 Kit ELISA, GB16111 Kit ELISA, hm:zehp0384 Kit ELISA, wu:fa95e06 Kit ELISA, wu:fb08g07 Kit ELISA, wu:fd05d08 Kit ELISA, DDBDRAFT_0217704 Kit ELISA, DDBDRAFT_0231192 Kit ELISA, DDB_0217704 Kit ELISA, DDB_0231192 Kit ELISA, ribosomal protein L13A Kit ELISA, ribosomal protein L13a Kit ELISA, Ribosomal protein L13A Kit ELISA, ribosomal protein L13a S homeolog Kit ELISA, 60S ribosomal protein L13a Kit ELISA, ribosomal protein 13a Kit ELISA, ribosomal 60S subunit protein L13A Kit ELISA, Ribosomal protein L13a, component of cytosolic 80S ribosome and 60S large subunit Kit ELISA, S60 ribosomal protein L13a Kit ELISA, Rpl13a Kit ELISA, RPL13A Kit ELISA, RpL13A Kit ELISA, rpl13a.S Kit ELISA, rpl13a Kit ELISA, LOC551418 Kit ELISA, LOC663151 Kit ELISA, RPL13a Kit ELISA

Sujet:

Gene Name: Ribosomal Protein L13A

Gene Aliases: TSTA1, Tissue Specific Transplantation Antigen 1, 60S ribosomal protein L13a, 23 kDa highly basic protein