RBM38 Kit ELISA

N° du produit ABIN5658527

Détail du produit RBM38 Kit ELISA

Antigène: RBM38 (RNA Binding Motif Protein 38 (RBM38))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of RNA Binding Motif Protein 38 (RBM38). No significant cross-reactivity or interference between RNA Binding Motif Protein 38 (RBM38) and analogues was observed.

Sensibilité: 0.112 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNA Binding Motif Protein 38 (RBM38). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RNA Binding Motif Protein 38 (RBM38). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNA Binding Motif Protein 38 (RBM38), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RNA Binding Motif Protein 38 (RBM38) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RNA Binding Motif Protein 38 (RBM38) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RNA Binding Motif Protein 38 (RBM38) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RBM38 (RNA Binding Motif Protein 38 (RBM38))

Autre désignation: RNA Binding Motif Protein 38 (RBM38 Produits)

Synonymes: rnpc1 Kit ELISA, seb4b Kit ELISA, seb4d Kit ELISA, XSeb4R Kit ELISA, MGC89204 Kit ELISA, hsrnaseb Kit ELISA, YF-55 Kit ELISA, fi25e12 Kit ELISA, wu:fi25e12 Kit ELISA, zgc:92336 Kit ELISA, xseb4r Kit ELISA, HSRNASEB Kit ELISA, RNPC1 Kit ELISA, SEB4B Kit ELISA, SEB4D Kit ELISA, dJ800J21.2 Kit ELISA, Rnpc1 Kit ELISA, Seb4 Kit ELISA, Seb4l Kit ELISA, RNA binding motif protein 38 Kit ELISA, RNA binding motif protein 38 L homeolog Kit ELISA, rbm38 Kit ELISA, rbm38.L Kit ELISA, RBM38 Kit ELISA, Rbm38 Kit ELISA

Sujet:

Gene Name: RNA Binding Motif Protein 38

Gene Aliases: HSRNASEB, SEB4D, Seb4B, RNPC1, RNA-Binding Region(RNP1,RRM)Containing 1, CLL-associated antigen KW-5, ssDNA-binding protein SEB4

ID gène: 55544

UniProt: Q9H0Z9

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development