ROBO1 Kit ELISA

N° du produit ABIN5658530

Détail du produit ROBO1 Kit ELISA

Antigène: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1). No significant cross-reactivity or interference between Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) and analogues was observed.

Sensibilité: 0.118 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Autre désignation: Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1 Produits)

Synonymes: DUTT1 Kit ELISA, SAX3 Kit ELISA, AW494633 Kit ELISA, AW742721 Kit ELISA, Gm310 Kit ELISA, ROBO1 Kit ELISA, dutt1 Kit ELISA, roundabout Kit ELISA, sax3 Kit ELISA, MGC122264 Kit ELISA, roundabout-1 Kit ELISA, roundabout guidance receptor 1 Kit ELISA, roundabout, axon guidance receptor, homolog 1 (Drosophila) Kit ELISA, roundabout guidance receptor 1 L homeolog Kit ELISA, ROBO1 Kit ELISA, Robo1 Kit ELISA, robo1 Kit ELISA, robo1.L Kit ELISA

Sujet:

Gene Name: Roundabout, Axon Guidance Receptor, Homolog 1

Gene Aliases: DUTT1, SAX3, Roundabout homolog 1, Deleted in U twenty twenty, H-Robo-1

Pathways: Positive Regulation of Endopeptidase Activity