SKP1 Kit ELISA

N° du produit ABIN5658539

Détail du produit SKP1 Kit ELISA

Antigène: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of S-Phase Kinase Associated Protein 1 (SKP1). No significant cross-reactivity or interference between S-Phase Kinase Associated Protein 1 (SKP1) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-Phase Kinase Associated Protein 1 (SKP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S-Phase Kinase Associated Protein 1 (SKP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S-Phase Kinase Associated Protein 1 (SKP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S-Phase Kinase Associated Protein 1 (SKP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S-Phase Kinase Associated Protein 1 (SKP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S-Phase Kinase Associated Protein 1 (SKP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Autre désignation: S-Phase Kinase Associated Protein 1 (SKP1 Produits)

Synonymes: EMC19 Kit ELISA, OCP-II Kit ELISA, OCP2 Kit ELISA, SKP1A Kit ELISA, TCEB1L Kit ELISA, p19A Kit ELISA, 15kDa Kit ELISA, 2610043E24Rik Kit ELISA, 2610206H23Rik Kit ELISA, SKP1 Kit ELISA, Tceb1l Kit ELISA, p19Skp1 Kit ELISA, Skp1a Kit ELISA, skp1a Kit ELISA, zgc:73186 Kit ELISA, ocp2 Kit ELISA, emc19 Kit ELISA, ocp-ii Kit ELISA, tceb1l Kit ELISA, ARABIDOPSIS SKP1 HOMOLOGUE 1 Kit ELISA, ASK1 Kit ELISA, ATSKP1 Kit ELISA, S phase kinase-associated protein 1 Kit ELISA, T4O12.17 Kit ELISA, T4O12_17 Kit ELISA, UFO INTERACTING PROTEIN 1 Kit ELISA, UIP1 Kit ELISA, ocp-2 Kit ELISA, S-phase kinase associated protein 1 Kit ELISA, S-phase kinase-associated protein 1 Kit ELISA, S-phase kinase-associated protein 1 pseudogene Kit ELISA, S-phase kinase-associated protein 1A Kit ELISA, S-phase kinase-associated protein 1 S homeolog Kit ELISA, S phase kinase-associated protein 1 Kit ELISA, SKP1 Kit ELISA, skp1 Kit ELISA, LOC100340043 Kit ELISA, Skp1a Kit ELISA, Skp1 Kit ELISA, skp1.S Kit ELISA

Sujet:

Gene Name: S-Phase Kinase Associated Protein 1

Gene Aliases: SKP1A, EMC19, OCP-II, OCP2, TCEB1L, p19A, Cyclin-A/CDK2-associated p19, RNA polymerase II elongation factor-like protein, Transcription elongation factor B polypeptide 1-like

ID gène: 6500

UniProt: P63208

Pathways: Cycle Cellulaire, Signalisation Hedgehog, Mitotic G1-G1/S Phases