S100A10 Kit ELISA

N° du produit ABIN5658548

Détail du produit S100A10 Kit ELISA

Antigène: S100A10 (S100 Calcium Binding Protein A10 (S100A10))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A10 (S100A10). No significant cross-reactivity or interference between S100 Calcium Binding Protein A10 (S100A10) and analogues was observed.

Sensibilité: 0.115 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A10 (S100A10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A10 (S100A10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A10 (S100A10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A10 (S100A10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A10 (S100A10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A10 (S100A10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: S100A10 (S100 Calcium Binding Protein A10 (S100A10))

Autre désignation: S100 Calcium Binding Protein A10 (S100A10 Produits)

Synonymes: 42C Kit ELISA, ANX2L Kit ELISA, ANX2LG Kit ELISA, CAL1L Kit ELISA, CLP11 Kit ELISA, Ca[1] Kit ELISA, GP11 Kit ELISA, P11 Kit ELISA, p10 Kit ELISA, AA409961 Kit ELISA, AL024248 Kit ELISA, CAL12 Kit ELISA, Cal1l Kit ELISA, p11 Kit ELISA, s100a10 Kit ELISA, 42c Kit ELISA, anx2l Kit ELISA, anx2lg Kit ELISA, cal1l Kit ELISA, calpactin Kit ELISA, clp11 Kit ELISA, gp11 Kit ELISA, p11-A Kit ELISA, s100a10b Kit ELISA, hm:zehn1116 Kit ELISA, wu:fa94d11 Kit ELISA, wu:fb05h04 Kit ELISA, zgc:56142 Kit ELISA, zgc:101880 Kit ELISA, S100 calcium binding protein A10 Kit ELISA, S100 calcium binding protein A10 (calpactin) Kit ELISA, S100 calcium binding protein A10 L homeolog Kit ELISA, S100 calcium binding protein A10b Kit ELISA, S100 calcium binding protein A10a Kit ELISA, S100A10 Kit ELISA, S100a10 Kit ELISA, s100a10 Kit ELISA, s100a10.L Kit ELISA, s100a10b Kit ELISA, s100a10a Kit ELISA

Sujet:

Gene Name: S100 Calcium Binding Protein A10

Gene Aliases: ANX2L, ANX2LG, CAL1L, CLP11, GP11, P11, P10, Calpactin-1 light chain, Cellular ligand of annexin II, Annexin II Ligand,Calpactin I,Light Polypeptide

UniProt: P05943

Pathways: S100 Proteins