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s100a4 Kit ELISA

S100A4 Reactivité: Humain Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5658560
  • Antigène Voir toutes s100a4 (S100A4) Kits ELISA
    s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
    Reactivité
    • 5
    • 2
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78 pg/mL - 5000 pg/mL
    Seuil minimal de détection
    78 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A4 (S100A4). No significant cross-reactivity or interference between S100 Calcium Binding Protein A4 (S100A4) and analogues was observed.
    Sensibilité
    30 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A4 (S100A4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A4 (S100A4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A4 (S100A4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A4 (S100A4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A4 (S100A4) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A4 (S100A4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes s100a4 (S100A4) Kits ELISA
    s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
    Autre désignation
    S100 Calcium Binding Protein A4 (S100A4 Produits)
    Synonymes
    18A2 Kit ELISA, 42A Kit ELISA, CAPL Kit ELISA, FSP1 Kit ELISA, MTS1 Kit ELISA, P9KA Kit ELISA, PEL98 Kit ELISA, 42a Kit ELISA, Capl Kit ELISA, FSp1 Kit ELISA, Mts1 Kit ELISA, PeL98 Kit ELISA, metastasin Kit ELISA, pk9a Kit ELISA, S100A4 Kit ELISA, P9ka Kit ELISA, RNP9KA Kit ELISA, S100 calcium binding protein A4 Kit ELISA, S100 calcium-binding protein A4 Kit ELISA, S100A4 Kit ELISA, S100a4 Kit ELISA
    Sujet

    Gene Name: S100 Calcium Binding Protein A4

    Gene Aliases: S100-A4, MTS1, 18A2, 42A, CAPL, P9KA, PEL98, Calcium protein, Calvasculin, Metastasin, Protein Mts1, Placental calcium-binding protein, Murine placental homolog

    ID gène
    6275
    UniProt
    P26447
    Pathways
    S100 Proteins
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