S100A6 Kit ELISA
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- Antigène Voir toutes S100A6 Kits ELISA
- S100A6 (S100 Calcium Binding Protein A6 (S100A6))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 78 pg/mL - 5000 pg/mL
- Seuil minimal de détection
- 78 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A6 (S100A6). No significant cross-reactivity or interference between S100 Calcium Binding Protein A6 (S100A6) and analogues was observed.
- Sensibilité
- 30 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A6 (S100A6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A6 (S100A6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A6 (S100A6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A6 (S100A6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A6 (S100A6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A6 (S100A6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes S100A6 Kits ELISA
- S100A6 (S100 Calcium Binding Protein A6 (S100A6))
- Autre désignation
- S100 Calcium Binding Protein A6 (S100A6 Produits)
- Synonymes
- S100A6 Kit ELISA, 2A9 Kit ELISA, 5B10 Kit ELISA, CABP Kit ELISA, CACY Kit ELISA, PRA Kit ELISA, CALCYCLIN Kit ELISA, Cacy Kit ELISA, S100 calcium binding protein A6 Kit ELISA, uncharacterized LOC618256 Kit ELISA, S100 calcium binding protein A6 (calcyclin) Kit ELISA, S100A6 Kit ELISA, S100a6 Kit ELISA, LOC618256 Kit ELISA
- Sujet
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Gene Name: S100 Calcium Binding Protein A6
Gene Aliases: S100-A6, 2A9, 5B10, CABP, CACY, PRA, MLN 4, Calcyclin, Growth factor-inducible protein 2A9, Prolactin receptor-associated protein
- ID gène
- 6277
- UniProt
- P06703
- Pathways
- S100 Proteins
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