SEMG1 Kit ELISA

N° du produit ABIN5658708

Détail du produit SEMG1 Kit ELISA

Antigène: SEMG1 (Semenogelin I (SEMG1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Seminal Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Semenogelin I (SEMG1). No significant cross-reactivity or interference between Semenogelin I (SEMG1) and analogues was observed.

Sensibilité: 6.7 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semenogelin I (SEMG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semenogelin I (SEMG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semenogelin I (SEMG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semenogelin I (SEMG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semenogelin I (SEMG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semenogelin I (SEMG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SEMG1 (Semenogelin I (SEMG1))

Autre désignation: Semenogelin I (SEMG1 Produits)

Synonymes: SEMG1 Kit ELISA, SEMG1a Kit ELISA, CT103 Kit ELISA, SEMG Kit ELISA, SGI Kit ELISA, dJ172H20.2 Kit ELISA, RATSVPIIA Kit ELISA, SVPIIA Kit ELISA, SVS2P Kit ELISA, Svp1 Kit ELISA, Svs2 Kit ELISA, Svs2p2 Kit ELISA, BB115391 Kit ELISA, Semg1 Kit ELISA, SvsII Kit ELISA, semenogelin II Kit ELISA, semenogelin I Kit ELISA, semenogelin-2 Kit ELISA, seminal vesicle secretory protein 2 Kit ELISA, SEMG2 Kit ELISA, SEMG1 Kit ELISA, LOC100408385 Kit ELISA, Semg1 Kit ELISA, Svs2 Kit ELISA

Sujet:

Gene Name: Semenogelin I

Gene Aliases: SEMG, SGI, CT103, Semen Coagulating Protein, Cancer/Testis Antigen 103, Seminal basic protein

ID gène: 6406

UniProt: P04279